Engineering human stellate cells for beta cell replacement therapy promotes in vivo recruitment of regulatory T cells.

Type 1 diabetes (T1D) is an autoimmune illness characterised by destruction of pancreatic β cells. One of the promising therapeutic approaches in T1D is the transplantation of islets; nevertheless, it has critical limitations.

To tackle these limitations, immunotherapeutic methods have targeted on restoring immunologic tolerance, stopping transplanted cell destruction by sufferers’ personal immune system. Macrophage-derived chemokines comparable to chemokine-ligand-22 (CCL22) will be utilized for regulatory T cell (Treg) recruitment and graft tolerance.

Stellate cells (SCs) have numerous immunomodulatory features: recruitment of Tregs and induction of T-cell apoptosis. Here, we designed a novel immune-privileged microenvironment round implantable islets by overexpression of CCL22 proteins by SCs. We ready pseudoislets with insulin-secreting mouse insulinoma-6 (MIN6) cells and human SCs as a mannequin to imitate naive islet morphology. Our outcomes demonstrated that transduced SCs can secrete CCL22 and recruit Tregs towards ​the implantation web site in vivo.

Engineering human stellate cells for beta cell replacement therapy promotes in vivo recruitment of regulatory T cells.
Engineering human stellate cells for beta cell replacement therapy promotes in vivo recruitment of regulatory T cells.

This research is promising to offer a basic understanding of SC-islet interplay and ligand synthesis and transport from SCs on the graft web site for guaranteeing native immune tolerance. Our outcomes additionally set up a brand new paradigm for creating tolerable grafts for different persistent ailments comparable to diabetes, anemia, and central nervous system (CNS) ailments, and advance the science of graft tolerance.

Dcaf7 antibody

70R-9146 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal Dcaf7 antibody

DCAF7 Antibody

1-CSB-PA026056ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DCAF7. Recognizes DCAF7 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

DCAF7 Antibody

1-CSB-PA026056GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against DCAF7. Recognizes DCAF7 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC

DCAF7 Rabbit pAb

A13392-100ul 100 ul
EUR 369.6

DCAF7 Rabbit pAb

A13392-200ul 200 ul
EUR 550.8

DCAF7 Rabbit pAb

A13392-20ul 20 ul
EUR 219.6

DCAF7 Rabbit pAb

A13392-50ul 50 ul
EUR 267.6

Dcaf7 Blocking Peptide

33R-8588 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Dcaf7 antibody, catalog no. 70R-9146

DCAF7 Rabbit pAb

A6787-100ul 100 ul
EUR 369.6

DCAF7 Rabbit pAb

A6787-200ul 200 ul
EUR 550.8

DCAF7 Rabbit pAb

A6787-20ul 20 ul
EUR 219.6

DCAF7 Rabbit pAb

A6787-50ul 50 ul
EUR 267.6

DCAF7 Conjugated Antibody

C39181 100ul
EUR 476.4

Anti-DCAF7 antibody

STJ115354 100 µl
EUR 332.4
Description: This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants.

Anti-DCAF7 antibody

STJ28870 100 µl
EUR 332.4
Description: This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants.

DCAF7 protein (His tag)

80R-1937 100 ug
EUR 386.4
Description: Recombinant human DCAF7 protein

Human DCAF7 shRNA Plasmid

20-abx956925
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse DCAF7 shRNA Plasmid

20-abx977535
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

DCAF7 Recombinant Protein (Rat)

RP197414 100 ug Ask for price

DCAF7 Recombinant Protein (Human)

RP054231 100 ug Ask for price

DCAF7 Recombinant Protein (Mouse)

RP128021 100 ug Ask for price

Dcaf7 ORF Vector (Rat) (pORF)

ORF065806 1.0 ug DNA
EUR 607.2

DCAF7 ORF Vector (Human) (pORF)

ORF018078 1.0 ug DNA Ask for price

Dcaf7 ORF Vector (Mouse) (pORF)

ORF042675 1.0 ug DNA
EUR 607.2

Polyclonal Dcaf7 antibody - N-terminal region

APR01129G 0.05mg
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Dcaf7 - N-terminal region. This antibody is tested and proven to work in the following applications:

Dcaf7 sgRNA CRISPR Lentivector set (Rat)

K6329601 3 x 1.0 ug
EUR 406.8

DCAF7 sgRNA CRISPR Lentivector set (Human)

K2763301 3 x 1.0 ug
EUR 406.8

Dcaf7 sgRNA CRISPR Lentivector set (Mouse)

K3492801 3 x 1.0 ug
EUR 406.8

Dcaf7 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6329602 1.0 ug DNA
EUR 184.8

Dcaf7 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6329603 1.0 ug DNA
EUR 184.8

Dcaf7 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6329604 1.0 ug DNA
EUR 184.8

DCAF7 sgRNA CRISPR Lentivector (Human) (Target 1)

K2763302 1.0 ug DNA
EUR 184.8

DCAF7 sgRNA CRISPR Lentivector (Human) (Target 2)

K2763303 1.0 ug DNA
EUR 184.8

DCAF7 sgRNA CRISPR Lentivector (Human) (Target 3)

K2763304 1.0 ug DNA
EUR 184.8

Dcaf7 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3492802 1.0 ug DNA
EUR 184.8

Dcaf7 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3492803 1.0 ug DNA
EUR 184.8

Dcaf7 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3492804 1.0 ug DNA
EUR 184.8

DCAF7 Protein Vector (Human) (pPB-C-His)

PV072309 500 ng Ask for price

DCAF7 Protein Vector (Human) (pPB-N-His)

PV072310 500 ng Ask for price

DCAF7 Protein Vector (Human) (pPM-C-HA)

PV072311 500 ng Ask for price

DCAF7 Protein Vector (Human) (pPM-C-His)

PV072312 500 ng Ask for price

DCAF7 Protein Vector (Mouse) (pPB-C-His)

PV170698 500 ng
EUR 723.6

DCAF7 Protein Vector (Mouse) (pPB-N-His)

PV170699 500 ng
EUR 723.6

DCAF7 Protein Vector (Mouse) (pPM-C-HA)

PV170700 500 ng
EUR 723.6

DCAF7 Protein Vector (Mouse) (pPM-C-His)

PV170701 500 ng
EUR 723.6

DCAF7 3'UTR Luciferase Stable Cell Line

TU005593 1.0 ml
EUR 2799.6

Dcaf7 3'UTR GFP Stable Cell Line

TU253183 1.0 ml Ask for price

Dcaf7 3'UTR Luciferase Stable Cell Line

TU104884 1.0 ml Ask for price

DCAF7 3'UTR GFP Stable Cell Line

TU055593 1.0 ml
EUR 2799.6

Dcaf7 3'UTR Luciferase Stable Cell Line

TU203183 1.0 ml Ask for price

Dcaf7 3'UTR GFP Stable Cell Line

TU154884 1.0 ml Ask for price

DCAF7 Protein Vector (Rat) (pPB-C-His)

PV263222 500 ng
EUR 723.6

DCAF7 Protein Vector (Rat) (pPB-N-His)

PV263223 500 ng
EUR 723.6

DCAF7 Protein Vector (Rat) (pPM-C-HA)

PV263224 500 ng
EUR 723.6

DCAF7 Protein Vector (Rat) (pPM-C-His)

PV263225 500 ng
EUR 723.6

DDB1 And CUL4 Associated Factor 7 (DCAF7) Antibody

20-abx141912
  • EUR 444.00
  • EUR 727.20
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

DDB1 And CUL4 Associated Factor 7 (DCAF7) Antibody

20-abx005207
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DDB1 And CUL4 Associated Factor 7 (DCAF7) Antibody

20-abx116663
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

DDB1 And CUL4 Associated Factor 7 (DCAF7) Antibody

20-abx320404
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

Human DDB1- and CUL4-associated factor 7 (DCAF7)

1-CSB-EP026056HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human DDB1- and CUL4-associated factor 7(DCAF7) expressed in E.coli

Human DDB1- and CUL4- associated factor 7, DCAF7 ELISA KIT

ELI-47000h 96 Tests
EUR 988.8

Mouse DDB1- and CUL4- associated factor 7, Dcaf7 ELISA KIT

ELI-32390m 96 Tests
EUR 1038

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6329605 3 x 1.0 ug
EUR 451.2

DCAF7 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K2763305 3 x 1.0 ug
EUR 451.2

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3492805 3 x 1.0 ug
EUR 451.2

DCAF7 DDB1 and CUL4 Associated Factor 7 Human Recombinant Protein

PROTP61962 Regular: 20ug
EUR 380.4
Description: DCAF7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (19-295 a.a) and having a molecular mass of 33.6kDa.;DCAF7 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6329606 1.0 ug DNA
EUR 200.4

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6329607 1.0 ug DNA
EUR 200.4

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6329608 1.0 ug DNA
EUR 200.4

DCAF7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2763306 1.0 ug DNA
EUR 200.4

DCAF7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K2763307 1.0 ug DNA
EUR 200.4

DCAF7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K2763308 1.0 ug DNA
EUR 200.4

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3492806 1.0 ug DNA
EUR 200.4

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3492807 1.0 ug DNA
EUR 200.4

Dcaf7 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3492808 1.0 ug DNA
EUR 200.4

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